Arginase plays an important role in ammonia detoxification of yellow catfish Pelteobagrus fulvidraco

A two-stage study was carried out to test the mechanism of arginase in ammonia detoxification yellow catfish. At stage 1, fish injected lethal half concentration ammonium acetate and 0.9% sodium chloride respectively every 12 h six replicates for 72 h. The result found that no significant different serum contents group at hours 12, 24, 36, 48, 60 72. 2, split two, one continued with (NH3 group) other valine (an inhibitor arginase; Val group); Sodium also (NaCl + group). experiment Serum liver arginine were higher than those NH3 group; Compared NaCl group, activity ARG 1 expression lower; Fish groups had lowest superoxide dismutase activities, malondialdehyde, tumor necrosis factor-α, interleukin 8 contents, TNF-α, IL-1 IL-8 expressions groups, lysozyme complement 3 4 contents. This indicates poisoning would lead oxidative damage, immunosuppression inflammation catfish; Arginase may be an important target toxicity Exogenous supplementation might alleviate symptoms